LN-niche
Chuang Dong
Bioinformatics Scientist (IE INSERM)
Nadine Varin-Blank
Unit Director (DR INSERM)
Elisabetta Dondi
Research Engineer (IR USPN)
Implication of the lymph node tolerogenic niche in the progression of Chronic Lymphocytic Leukemia (CLL)
Research Overview
- We aim to perform a complete characterization of the cellular and molecular compositions of lymph node biopsies from CLL patients exhibiting progressive loss of structural organization in order to understand the mechanisms involved in the shift from a “normal” cellular microenvironment to a pro-CLL survival microenvironment. We plan to simultaneously detect specific markers, immunoregulatory factors at the protein and mRNA levels to obtain a phenotypic identification and the spatial distribution of cells, along with characterization of the interactions between cell populations
- We wish to further characterize the bidirectional cross-talk between CLL B cells and NLCs. We observed that CLL cells might exhibit different potential to induce NLC differentiation which call to identify the critical signals from CLL B cells inducing M2 differentiation. In parallel, we analyze both membrane and soluble effectors supporting CLL cells survival and trafficking. We also evaluate the impact of therapeutic agents on the expression of these factors.
